Freeze Fracturing of Hot Samples
نویسندگان
چکیده
منابع مشابه
Freeze fracturing of elastic porous media: a mathematical model.
We present a mathematical model of the fracturing of water-saturated rocks and other porous materials in cold climates. Ice growing inside porous rocks causes large pressures to develop that can significantly damage the rock. We study the growth of ice inside a penny-shaped cavity in a water-saturated porous rock and the consequent fracturing of the medium. Premelting of the ice against the roc...
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That synaptic vesicles with a diameter of about 50 nm could be demonstrated by the freeze fracturing technique was an important contribution to the knowledge of synaptic fine structure (Moor, Pfenninger & Akert 1969; Akert, Moor, Pfenninger & Sandri 1969). The finding o spheric profiles (figures 1, 3 a, c) in rapidly frozen tissue renders an artefactual origin of vesicles very unlikely. Howeve...
متن کاملFreeze-fracturing of Nerve Growth Cones and Young Fibers
Neural and non-neural cellular processes have been studied in organotypic cultures of spinal cord and olfactory bulb by means of the freeze-fracturing technique. Identification of specific cellular elements in replicas has been achieved by comparison with thin-sectioned material in which differences in shape and contents are evident. Freeze-fracturing reveals that neural growth cones may be dis...
متن کاملFreeze-fracturing in ultrahigh vacuum at -196 degrees C
Conventional freeze-etching is carried out in a vacuum of approximately 10(-6) torr and at a specimen temperature of -100 degrees C. The relatively poor topographic resolution of most freeze-etch replicas, and the lack of complementarity of morphological details in double replicas have been thought to be caused by structural distortions during fracturing, and radiation damage during replication...
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The fine structure of the regular arrays of subunits seen on both plasmalemma fracture faces in resting and starved Saccharomyces cerevisiae (baker's yeast) has been compared using different freeze-fracture replication methods. Freeze-cleaving was carried out at 173 degrees, 133 degrees, and 108 degrees K under a vacuum of 2 X 10(-7) torr (2.6 X 10(- 7)mbar) or under liquid nitrogen at atmosphe...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1997
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500056066